Ilmira Rinatovna Gilmutdinova

Study on fibroblasts, mesenchymal and immunocompetent cells.

The biocompatibility of the investigational product is assessed as part of the experiment:

• effect on cell viability (toxicity)
• influence on the proliferative activity of cells (doubling rate)
• determination of phenotypic changes in cells (phenotype))
• quantification of growth factors and cytokines produced by cells

Methods in use:

• Cell phenotype at each seeding/transfection (flow cytofluorimeter)
• Cell counting at each seeding/transfer
• Photo documentation of cells
• Giemsa staining
• Luminescence microscopy
• Scanning electron microscopy
• Enzyme-linked immunosorbent assay
• Cell activity study on a spectrophotometer

Study on white laboratory rats (number, sex, weight to be agreed on separately).

Methods in use:

• General and extended blood chemistry
• NBT test
• Luminescence microscopy
• Electron microscopy
• General morphological research methods

This is an instrument that allows rapid assessment of cell population composition by fluorescence and optical characteristics of cells. It can be used to determine the absolute and relative number of cells of different populations and subpopulations. The cytofluorimeter can analyse both fixed and living cells with a diameter ranging between 0.5 µm and 40 µm.

Medical and biological studies examine samples prepared from the cells of:

• bone marrow;
• blood;
• cerebrospinal fluid (ceneurolymph);
• synovial (joint) fluid;
• pleural fluid;
• peritoneal (ascitic) fluid that accumulates in the abdominal cavity during the development of ascites;
• tumour and healthy tissues.